Biotinoyl Tripeptide-1

Biotinoyl Tripeptide-1 is a synthetic conjugate consisting of biotin (vitamin B7, also historically called vitamin H) attached via an amide bond to the N-terminus of the GHK tripeptide (Gly-His-Lys), the same core tripeptide sequence found in GHK-Cu and Palmitoyl Tripeptide-1, both also built on this site. It is sold under several manufacturer-specific trade names, including ActiTide-BT1, SpecPed BT1P, and Cosroma BT-1, reflecting a cosmetic ingredient that, unlike some of the more tightly single-manufacturer-controlled peptides in this catalog (such as Sederma's Argireline or Leuphasyl), appears to be produced by multiple manufacturers under different brand names using the same underlying CAS-registered chemical structure.

At Verified Peptides, we want to directly name a pattern we have now observed three separate times in this catalog: the GHK tripeptide (Gly-His-Lys) is a common structural starting point for cosmetic ingredient development, with different chemical groups attached to modify its properties — a copper ion for GHK-Cu, a palmitic acid (fatty acid) chain for Palmitoyl Tripeptide-1, and biotin for Biotinoyl Tripeptide-1. Each of these three compounds is a genuinely distinct chemical entity with its own CAS number and, to varying degrees, its own separate evidence base, despite sharing the identical three-amino-acid backbone.

Chemically, Biotinoyl Tripeptide-1 has molecular formula C24H38N8O6S, molecular weight approximately 566.67 g/mol, CAS number 299157-54-3, PubChem CID 11534242. We independently cross-calculated this formula and molecular weight from first principles — summing the constituent formulas of biotin and the GHK tripeptide, then subtracting one water molecule for the amide-bond condensation joining them — and confirmed it reproduces the vendor-reported figures exactly.

Specifically, biotin (free acid, C10H16N2O3S) and the GHK tripeptide (assembled from glycine, histidine, and lysine with two peptide-bond condensations removing two water molecules, yielding C14H24N6O4) combine via a third condensation reaction — biotin's carboxylic acid group forming an amide bond with the GHK tripeptide's N-terminal amine — removing one additional water molecule. Summing the two component formulas (C24H40N8O7S) and subtracting this final water molecule yields C24H38N8O6S, matching the vendor-reported formula exactly, and a molecular weight of approximately 566.68 g/mol by atomic summation, closely matching the reported 566.67 g/mol.

At Verified Peptides, we want to be fully transparent that we could not locate any independently peer-reviewed, PubMed-indexed study on Biotinoyl Tripeptide-1 specifically. A direct PubMed search for 'biotinoyl tripeptide' returned zero results. All of the specific mechanistic claims we found for this compound — hair bulb keratinocyte proliferation, laminin 5 and collagen IV synthesis and organization — trace to manufacturer in vitro technical literature (cell-culture assay data provided by the ingredient's commercial suppliers), not to independently published, peer-reviewed academic research that we could verify.

This places Biotinoyl Tripeptide-1 among the thinner independently-verified evidence bases in this catalog, alongside Myristoyl Pentapeptide-17, though with an important difference worth noting: unlike Myristoyl Pentapeptide-17 (where we found one real, if limited, PubMed-indexed clinical study), we could not identify any independently published study of any kind — controlled, uncontrolled, in vitro, or in vivo — specifically testing Biotinoyl Tripeptide-1, whether alone or in combination with other ingredients. We want to be equally clear that the GHK tripeptide core itself has substantial independent research support in its copper-chelated form (GHK-Cu, also built on this site), but this existing GHK-Cu research should not be assumed to transfer to Biotinoyl Tripeptide-1's different, biotin-conjugated chemistry, since the two compounds have different structures and, plausibly, different biological activity profiles despite sharing the GHK backbone.

No FDA, EMA, or other Western regulatory body evaluates Biotinoyl Tripeptide-1 as a drug, since it is regulated as a cosmetic ingredient. At Verified Peptides, we sell research-grade Biotinoyl Tripeptide-1 exclusively for laboratory research, and we think this compound is a useful reminder that being sold by multiple established cosmetic-ingredient manufacturers under several different trade names is not, by itself, evidence of independent scientific validation — commercial availability and scientific evidence are separate questions that we evaluate separately for every compound in this catalog.

Unlike several other cosmetic peptides in this catalog that are proprietary to a single originating manufacturer (Sederma's Argireline and Leuphasyl, Symrise's Myristoyl Pentapeptide-17), Biotinoyl Tripeptide-1 appears to be manufactured and sold under multiple independent trade names (ActiTide-BT1, SpecPed BT1P, Cosroma BT-1) by different suppliers, suggesting the compound's specific chemical structure (CAS 299157-54-3) may not be protected by the same kind of proprietary manufacturing exclusivity as some other cosmetic peptides in this catalog, which is itself an interesting general fact about how the cosmetic-ingredient supply chain works once a compound's basic structure becomes widely known and reproducible.

Biotin's independently well-documented role in human hair health is specific to correcting biotin deficiency, a nutritional state that is comparatively uncommon in individuals with adequate dietary intake; supplementing biotin (whether orally or via a topical biotin-conjugated peptide) in a person who is not biotin-deficient has a separate and much less well-established evidence base for improving hair growth or thickness, a general point relevant well beyond this single compound.

Laminin 5 (more formally known as laminin-332 in current nomenclature) and collagen IV are both genuine, well-studied basement membrane components broadly important across many tissues, not unique to hair follicles — laminin-332 mutations, for example, are independently known to cause a severe skin blistering disease (junctional epidermolysis bullosa) unrelated to any cosmetic application, illustrating that these are real structural proteins with an established broader biology, even though the specific claim that Biotinoyl Tripeptide-1 increases their synthesis in hair bulb keratinocytes remains manufacturer-sourced rather than independently confirmed.

This pattern — a genuinely well-studied underlying biological target (basement membrane adhesion proteins, in this case) paired with a specific commercial ingredient's claimed effect on that target that has not itself been independently confirmed — is one we have now seen repeatedly across this catalog's cosmetic peptides, and we think naming it explicitly here is more useful to researchers than treating each instance as an isolated, unrelated finding specific only to this one compound.

At Verified Peptides, we require independent third-party HPLC purity testing and mass spectrometry identity confirmation for every batch of research-grade Biotinoyl Tripeptide-1 we offer, with a Certificate of Analysis (COA) available for each lot. As a biotin-conjugated peptide, correct synthesis of the biotin-peptide amide bond should be confirmed as part of identity testing, not just the underlying GHK tripeptide sequence alone.

Because this compound is manufactured under several different trade names by different suppliers, researchers should confirm that any specific product's COA identifies the same CAS number (299157-54-3) and molecular formula (C24H38N8O6S) regardless of which trade name it is marketed under, since different manufacturers using different brand names for the same underlying chemical structure is a normal but sometimes confusing feature of the cosmetic-ingredient supply chain. We source exclusively from manufacturers operating under Good Manufacturing Practice (GMP)-aligned quality systems, and every lot is independently verified rather than accepted solely on a supplier's internal documentation.

Lyophilized Biotinoyl Tripeptide-1 is stable when stored at -20°C, protected from light and moisture. At Verified Peptides, we ship lyophilized peptide with appropriate cold-chain packaging to preserve stability in transit.

Once reconstituted, solution should be stored refrigerated at 2–8°C and used within the timeframe indicated on the product's documentation, avoiding repeated freeze-thaw cycles, consistent with standard handling practices for the other short synthetic peptides in this catalog.